[Histonet] F4/80 antibody

[Angelicola,Lisa]

I'm working on a research project that involves demonstrating
macrophages in formalin fixed paraffin embedded mouse skin wounds. The
researcher has purchased the F4/80 rat-anti-mouse antibody from abcam.
Has anyone out there in histoland had any experience working on fixed,
processed tissue with this antibody? The spec sheet states that it will
work with pretreatment of the tissue (protein digestion, which I would
like to avoid if possible since the tissue is susceptible to falling
off). Also where do you purchase your anti-rat secondary from? I would
appreciate any advice anyone might have as far as pretreatment,
dilutions, ect.
 
Thanks in advance,
 
Lisa Angelicola
Immunohistochemistry Lab
UCONN Health Center
Farmington, Ct.