[Histonet] Activated macrophages in porcine tissue (again!)

Y. Wang ynwang <@t> u.washington.edu
Fri Aug 6 10:37:10 CDT 2004


I am still looking for an antibody for activated macrophages that can be
used for immunohistochemistry in porcine tissue. I have contacted SEROTEC
as well as several other companies but without success and was wondering
if anyone had any ideas.

Just a brief background:
We want to identify resident and activated macrophages in porcine skin
samples (frozen sections). We already have a general macrophage label but
are having trouble finding specific ones.

Any other ideas on how we could distingush between the two would also be
greatly appreciated.

As a side note. Can anyone explain if ED1 or ED2 denotes activated
macrophages as the literature I have come across is very conflicted!

Thank you very much for your help
Yak-Nam Wang

Senior Fellow
Department of Bioengineering
University of Washington
Box 357962
Seattle, WA 98195

Tel.: (206)-221-5873
Fax.: (206)-221-5874

On Wed, 4 Aug 2004, Gayle Callis wrote:

> Contact SEROTEC, they have cross reaction charts to all kinds of species,
> including porcine.  If anything, they will do a search for you.
> At 10:33 AM 8/4/2004, you wrote:
> >Does anyone know of an antibody that can be used to label (IHC) activated
> >macrophages in porcine tissue?
> >
> >Thank you
> >
> >Yak-Nam Wang
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)

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