[Histonet] IF on frozen sections

[Gudrun Lang]

Thank you all for the answers! This list is really, really helpfull.
Gudrun

----- Original Message -----
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
To: "Gudrun Lang" <gudrun.lang <@t> aon.at>; "Histonetliste"
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 20, 2004 2:55 PM
Subject: RE: [Histonet] IF on frozen sections


I've always used OCT on my skins with no adverse effect.  It's so much
easier.
Happy cutting,

Juan

-----Original Message-----
From: Gudrun Lang [mailto:gudrun.lang <@t> aon.at]
Sent: Monday, April 19, 2004 11:34 AM
To: Histonetliste
Subject: [Histonet] IF on frozen sections


A question about immunfluorescence on frozen skin sections:
We mount the skin with buffer on the sample-table to freeze it. After
sectioning we let the cuts airdry and do the IF (Immunglobulins).
Is there any reason, why we cannot use OCT for mounting? I think sectioning
would be easier.
Perhaps someone can help me?

Lang Gudrun

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