[Histonet] IF on frozen sections

Tue Apr 20 07:55:17 CDT 2004

I've always used OCT on my skins with no adverse effect.  It's so much easier.  
Happy cutting,

Juan

-----Original Message-----
From: Gudrun Lang [mailto:gudrun.lang <@t> aon.at]
Sent: Monday, April 19, 2004 11:34 AM
To: Histonetliste
Subject: [Histonet] IF on frozen sections

A question about immunfluorescence on frozen skin sections:
We mount the skin with buffer on the sample-table to freeze it. After sectioning we let the cuts airdry and do the IF (Immunglobulins).
Is there any reason, why we cannot use OCT for mounting? I think sectioning would be easier.
Perhaps someone can help me?

Lang Gudrun

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