[Histonet] IF on frozen sections

[Mark Adam Tarango]

>>Date: Mon, 19 Apr 2004 18:33:35 +0200
>>From: "Gudrun Lang" <gudrun.lang <@t> aon.at>
>>Subject: [Histonet] IF on frozen sections
>>To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
>>Message-ID: <003301c4262c$0fe2b9f0$eeeea8c0 <@t> SERVER>
>>Content-Type: text/plain;	charset="iso-8859-1"

>>A question about immunfluorescence on frozen skin sections:
>>We mount the skin with buffer on the sample-table to freeze it. After
>>sectioning we let the cuts airdry and do the IF (Immunglobulins).
>>Is there any reason, why we cannot use OCT for mounting? I think
>>sectioning would be easier.
>>Perhaps someone can help me?

>>Lang Gudrun


I use OCT for cutting skin for IF staining.  My stains come out just
fine....



Mark Tarango