[Histonet] tissue slicer

Philip Oshel peoshel <@t> wisc.edu
Mon Apr 19 12:09:49 CDT 2004


Bad vibes, man.

But, Geoff, your device would work, but as you note, not for less 
than 1000 micron slices, definitely not 30 - 300 micron, and there'd 
be more tissue damage from compression. A vibratome is pretty much 
the only choice, there is no "all slices at once" instrument that 
I've seen.

Phil

>Always wondered what a vibratome was.
>I've got a wifatome.
>
>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
>  Consultant Pathologist
>  Rotherham General Hospital
>  South Yorkshire
>  England
>         terry.marshall <@t> rothgen.nhs.uk
>
>-----Original Message-----
>From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
>Sent: 19 April 2004 19:08
>To: k.whalley <@t> ich.ucl.ac.uk
>Cc: histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] tissue slicer
>
>
>Hi Katy:
>
>     You could buy a Vibratome, a device with a vibrating blade that will
>cut fixed or unfixed tissue at a thickness you select. I think there are
>several models and vendors.
>     Or, you could make an inexpensive device for little more than pocket
>change. Buy some high-quality double-edge razor blades and some material
>to use for spacing the blades. For 1 mm or more use square aluminum rod,
>for 0.5 mm or less use "shim stock". A well-stocked hardware store or
>maching shop will have these items. Use "super-glue" to glue up a
>"blade-spacer-blade-spacer-blade ..." tool with as many blades as your
>project demands. One 'application' of the tool to the sample will give
>you uniform and reproducable slices.  Be sure to  cut off or mask the
>edge of the blade not in use so you won't cut yourself.
>
>Geoff
>
>Katy Whalley wrote:
>
>>Hi,
>>
>>We are looking for a device which can be used to cut tissue quickly into
>>slices of an even thickness. We're not sure yet exactly how thick these
>>will be but something in the range 30-300 microns is likely. My supervisor
>>has in mind something in which several blades are attached to a holder
>>that keeps them the correct distance apart, so that all the slices are cut
>>at once. Has anyone ever used/ seen this kind of thing, or anything else
>>which would do the job?
>>
>>thanks,
>>Katy, UCL

-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)




More information about the Histonet mailing list