[Histonet] Best Marker for Proliferation?

[Jackie.O'Connor <@t> abbott.com]

My vote is with Ki67.  I work with human xenografts in mice, and you'll 
find in a lit search that Ki67 (MIB1) is the most commonly used marker for 
proliferation.  Most manufacturers antibodies work well  with HIER  - 
tough to find one that is human specific, tho - which means you get 
staining in the stroma, but for the most part it's inconsequential.

Jackie O'Connor





"Isaiah G. Schauer" <is135475 <@t> bcm.tmc.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/15/2004 10:41 PM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Best Marker for Proliferation?


Histonetters,

Good day. Thanks to all of you that replied to my previous message on 
CD31/PECAM-1 staining. I now have another question to pose.

What is the 'best' marker to use for immunohistochemical detection of 
cell proliferation on tissue slides from 4% paraformaldehyde-fixed, 
paraffin-embedded mouse xenograft tissue (human prostate epithelial 
cells mixed with mouse prostate stromal cells)? (Or, what is the most 
current, widely-accepted method)

The markers I know of are Ki67, PCNA, Cdc2 and Brdu-kits (non-tritiated 
thymidine method, ex: Roche cat 1 299 964), although I'm open to any 
other experienced suggestions.

Thanks for your time and advice.

Sincerely,
Isaiah Schauer
3rd year graduate student
Lab of David R. Rowley, Ph.D.
Baylor College of Medicine
Department of Molecular and Cellular Biology
One Baylor Plaza, room 326D
Houston, TX 77030

lab: 713-798-6221
fax: 713-790-1275


3rd year graduate student
Lab of David R. Rowley, Ph.D.
Baylor College of Medicine
Department of Molecular and Cellular Biology
One Baylor Plaza, room 326D
Houston, TX 77030

lab: 713-798-6221
fax: 713-790-1275


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