[Histonet] detach cells from a slide....again

Dolors Fuster d.fuster <@t> ub.edu
Wed Apr 14 11:50:23 CDT 2004


In my previous mail I forgot to tell you the mechanical method (scrape them off 
with a sharp razor blade) is made....but too slow for at least 600 
slides...difficult to recover all stuff...some of recovered adheres to the 
eppendorf.... :o(

Could the mehod not include trypsin in its procedure :o) ?? 

Thanks again



Any idea to detach cells from an hematologic slide?

They are dryed...stained with May Grünwald Giemsa...dehidrated, cleared and 
mounted with DPX.

The idea is to use them for a DNA extraction.

Thanks in advance



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----- Fi del missatge reenviat -----


Dolors Fuster
Tècnic Especialista en Anatomia Patològica i Citologia
Departament d'Anatomia i Embriologia Humana 
Facultat de Medicina (HCP) 
Universitat de Barcelona 
ICQ 14146798 



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