[Histonet] re perfusion debate

[Nancy.Walker <@t> sanofi-synthelabo.com]

Hello,

Back to an old perfusion debate...does anyone know the relation between the
pressure (mm Hg) and pump speed (ml/min) when doing a transcardiac
perfusion in mouse, that is, what pump speed will create a pressure of
approximately 300 mm Hg?

Charles, when you say to "start the prewash at low pressure and pump up to
300 mm Hg" what do you consider to be low pressure?

In the past I've done "slow" perfusions at 0.45 ml/min or "fast" perfusions
at 2ml/min, I would like to calculate the corresponding pressure. Any
physicists out there ...heeeeeeeeeeeeeeeeelp.

have a nice day!

Nancy Walker
Molecular Pharmacology

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker <@t> sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001