[Histonet] Antibody Source

[Stylli, Stanley]

Dear All,

Does anyone have any experience with using the following antibodies (with success) on rat tissue (especially brain) that has been formalin fixed and paraffin embedded :

Caspase-3
Tenascin-R
TNF-alpha
Vimentin
VEGF

I would prefer a monoclonal but am willing to purchase a polyclonal antibody from a commercial source.

Also, can you tell me if you managed to use the antibody with/without antigen retrieval.

Thanks

Stan