[Histonet] ISH on fresh frozen tissue

Tue Sep 30 08:57:11 CDT 2003

I am trying to do ISH on fresh frozen mouse testis. The morphology was o.k.
but my protocol doesn’t work. This material should not need pretreatment, I
suppose, although I must be wrong somewhere – it doesn’t work that way.
Pretreatment with protease E is very delicate. Has anybody got experience
he/she could share with me?
Thank you very much!!
Margaret

Margarete Vermehren
LMU München / Tieranatomie II
Tel:  +49 89 21805857
Fax: +49 89 21802569
m.vermehren <@t> anat.vetmed.uni-muenchen.de

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