<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
<meta name=ProgId content=Word.Document>
<meta name=Generator content="Microsoft Word 9">
<meta name=Originator content="Microsoft Word 9">
<link rel=File-List href="cid:filelist.xml@01C3876B.82263BA0">
<!--[if gte mso 9]><xml>
<o:OfficeDocumentSettings>
<o:DoNotRelyOnCSS/>
</o:OfficeDocumentSettings>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:WordDocument>
<w:View>Normal</w:View>
<w:Zoom>0</w:Zoom>
<w:DocumentKind>DocumentEmail</w:DocumentKind>
<w:HyphenationZone>21</w:HyphenationZone>
<w:EnvelopeVis/>
</w:WordDocument>
</xml><![endif]-->
<style>
<!--
/* Font Definitions */
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;
mso-font-charset:0;
mso-generic-font-family:swiss;
mso-font-pitch:variable;
mso-font-signature:16792199 0 0 0 65791 0;}
@font-face
{font-family:"Century Schoolbook";
panose-1:2 4 6 3 5 5 5 2 3 3;
mso-font-charset:0;
mso-generic-font-family:roman;
mso-font-pitch:variable;
mso-font-signature:7 0 0 0 147 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:EN-GB;}
p.MsoAutoSig, li.MsoAutoSig, div.MsoAutoSig
{margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:EN-GB;}
span.EmailFormatvorlage16
{mso-style-type:personal-compose;
mso-ansi-font-size:11.0pt;
mso-ascii-font-family:Tahoma;
mso-hansi-font-family:Tahoma;
color:black;
font-weight:normal;
font-style:normal;}
@page Section1
{size:612.0pt 792.0pt;
margin:72.0pt 90.0pt 72.0pt 90.0pt;
mso-header-margin:36.0pt;
mso-footer-margin:36.0pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
</style>
</head>
<body lang=DE style='tab-interval:35.4pt'>
<div class=Section1>
<p class=MsoNormal><span class=EmailFormatvorlage16><font size=2 color=black
face=Tahoma><span lang=EN-GB style='font-size:11.0pt;mso-bidi-font-size:12.0pt;
font-family:Tahoma;mso-bidi-font-family:"Times New Roman"'>I am trying to do
ISH on fresh frozen mouse testis. The morphology was o.k. but my protocol
doesn’t work. This material should not need pretreatment, I suppose, although I
must be wrong somewhere – it doesn’t work that way. Pretreatment with protease
E is very delicate. Has anybody got experience he/she could share with me?<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailFormatvorlage16><font size=2 color=black
face=Tahoma><span lang=EN-GB style='font-size:11.0pt;mso-bidi-font-size:12.0pt;
font-family:Tahoma;mso-bidi-font-family:"Times New Roman"'>Thank you very
much!!<o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailFormatvorlage16><font size=2 color=black
face=Tahoma><span lang=EN-GB style='font-size:11.0pt;mso-bidi-font-size:12.0pt;
font-family:Tahoma;mso-bidi-font-family:"Times New Roman"'>Margaret</span></font></span><span
class=EmailFormatvorlage16><font size=2 color=black face=Tahoma><span
style='font-size:11.0pt;mso-bidi-font-size:12.0pt;font-family:Tahoma;
mso-bidi-font-family:"Times New Roman";mso-ansi-language:DE'><o:p></o:p></span></font></span></p>
<p class=MsoNormal><span class=EmailFormatvorlage16><font size=2 color=black
face=Tahoma><span lang=EN-GB style='font-size:11.0pt;mso-bidi-font-size:12.0pt;
font-family:Tahoma;mso-bidi-font-family:"Times New Roman"'><![if !supportEmptyParas]> <![endif]><o:p></o:p></span></font></span></p>
<p class=MsoAutoSig><!--[if supportFields]><font color=black><span lang=EN-GB
style='color:black'><span style='mso-element:field-begin'></span></span></font><font
color=black><span style='color:black;mso-ansi-language:DE'><span
style="mso-spacerun: yes"> </span>AUTOTEXTLIST \s
"E-Mail-Signatur" </span><span lang=EN-GB><span style='mso-element:
field-separator'></span></span></font><![endif]--><font size=1 color=black
face="Century Schoolbook"><span style='font-size:9.0pt;mso-bidi-font-size:12.0pt;
font-family:"Century Schoolbook";color:black;mso-ansi-language:DE'>Margarete
Vermehren</span></font><font size=1 color=black face="Century Schoolbook"><span
style='font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";
color:black;mso-color-alt:windowtext;mso-ansi-language:DE'><o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=1 color=black face="Century Schoolbook"><span
style='font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";
color:black;mso-ansi-language:DE'>LMU München / Tieranatomie II</span></font><font
size=1 color=black face="Century Schoolbook"><span style='font-size:9.0pt;
mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";color:black;
mso-color-alt:windowtext;mso-ansi-language:DE'><o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=1 color=black face="Century Schoolbook"><span
lang=EN-GB style='font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";
color:black'>Tel:<span style="mso-spacerun: yes"> </span>+49 89 21805857</span></font><font
size=1 color=black face="Century Schoolbook"><span lang=EN-GB style='font-size:
9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";color:black;
mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=1 color=black face="Century Schoolbook"><span
lang=EN-GB style='font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";
color:black'>Fax: +49 89 21802569</span></font><font size=1 color=black
face="Century Schoolbook"><span lang=EN-GB style='font-size:9.0pt;mso-bidi-font-size:
12.0pt;font-family:"Century Schoolbook";color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=1 color=black face="Century Schoolbook"><span
lang=EN-GB style='font-size:9.0pt;mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";
color:black'>m.vermehren@anat.vetmed.uni-muenchen.de</span></font><font size=1
color=black face="Century Schoolbook"><span lang=EN-GB style='font-size:9.0pt;
mso-bidi-font-size:12.0pt;font-family:"Century Schoolbook";color:black;
mso-color-alt:windowtext'><o:p></o:p></span></font></p>
<p class=MsoNormal><!--[if supportFields]><font color=black><span lang=EN-GB
style='color:black'><span style='mso-element:field-end'></span></span></font><![endif]--><font
color=black><span lang=EN-GB style='color:black'><![if !supportEmptyParas]> <![endif]></span></font><font
color=black><span lang=EN-GB style='color:black;mso-color-alt:windowtext'><o:p></o:p></span></font></p>
</div>
</body>
</html>