[Histonet] Paraffin Embedding of Mice Tissue - My protocols

[Kelly Salceies]

Hi --

I guess I should have mentioned the protocols I have already tried -
sorry...I'm new to this Histonet thing :)

On the Shandon Hypercenter XP I've tried:

80% ETOH 5 min
95% ETOH 15 min 2X
100% ETOH 15 min 3X
Xylene  10 min 2X
Xylene 5 min 2X
Paraffin 15 min 2X

50% ETOH 5 min
80% ETOH 5 min
95% ETOH 15 min 2X
100% ETOH 15 min 3X
Xylene 10 min 2X
Xylene 5 min
Paraffin 15 min 2X

80% ETOH 5 min
95% ETOH 5 min 2X
100% ETOH 5 min 2X
1:1 ETOH: Cedarwood Oil 5 min
1:1 Cedarwood Oil:Methyl Salicylate 5 min
100% Methyl Salicylate 5 min
1:1 Methyl Salicylate:Xylene 5 min
Xylene 5min
Paraffin 15 min 2X

--The last protocol left the tissue soft - so I might just need to use
longer times...I'm not sure if anybody uses this protocol....

On the Microwave I've tried:

100% ETOH 10 min  2X
1:1 ETOH:Xylene 10 min
Paraffin 10 min    
-All steps: Max temp 65 degrees - Watt=750

100% ETOH 5 min
IPA 5 min
Paraffin 5 min
-All steps: Max temp 65 degrees - Watt=750

100% ETOH 5 min
1:1 IPA:Xylene 5 min
Paraffin 5 min
-All steps: Max temp 65 degrees - Watt=750


There are the protocols I've tried....Please let me know what
suggestions you have - thank you!!
Kelly Salceies
University of New Mexico
Health Sciences Center
Dept. of Pathology