[Histonet] Re: Brain Tissue in Buffer

[Charles W. Scouten, Ph.D.]

I have always heard you cannot unboil an egg, or unfix tissue.  

My guess is the brains were not fully fixed in the first place.  Fixation takes a minimum of four hours and continues to progress for up to 7 days in perfused brains.  Partial fixation may be best for immunological use.  But taking out of fixative before fully fixed and storing in PBS for a long tim may have an obvious result.   Partial detiorioration.  Were the brains in question fully fixed in the first place?

Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
www.myneurolab.com 


-----Original Message-----
From: Frouwke Kuijpers [mailto:frouwke <@t> sci.kun.nl] 
Sent: Thursday, September 25, 2003 3:39 AM
To: histonet <@t> lists.utsouthwestern.edu; Nancy.Walker <@t> sanofi-synthelabo.com
Subject: Re: [Histonet] Re: Brain Tissue in Buffer

My experience is that when fixed brain stays long in PBS, it becomes very
soft. Than I don't use these brains anymore.
Reason? I don't know. Somebody else maybe?

Frouwke Kuijpers

----- Original Message ----- 
From: <Nancy.Walker <@t> sanofi-synthelabo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 23, 2003 1:36 PM
Subject: [Histonet] Re: Brain Tissue in Buffer


>
> Hi,
> I've been told that if you fix in PFA then store in PBS for long periods
> the PBS "unfixes" the tissue. So I would suggest redoing your fixation
> process.
>
> Good luck!
>
>
>
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