[Histonet] Purpose of Oven Prior to Staining?

[Fred Underwood]

Hi Laurie.  I have found that with B5 fixed lymph nodes it is mandatory
to allow all water to evaporate from the section before heating.  It
seeemed that if the section was heated with water present, nuclear
detail was destroyed.  Almost like it became nuclear "soup" and the
morphology was terrible.

Fred

>>> Laurie Reilly <Laurie.Reilly <@t> jcu.edu.au> 09/18/03 07:34PM >>>
Dear Diane,
Your statement that"Ideally, the slides should be dried at
room temperature overnight especially if photographs are going to be
made"
fascinates me. Could you please explain the significance of room 
temperature drying
for photomicrography.

         Thanks and regards,  Laurie.

At 02:08 PM 09/18/03 -0400, Gladney, Diane C Ms MACH wrote:
>Jordan,
>
>The purpose of the slide dryer or oven is to dry the water off of the
>slides. The slides should be well drained to remove as much water as
>possible before placing them in the rack before it is placed in the
oven.
>It is not and will not deparaffinize the slides. The slides must be
>completely free of water before they are stained either by hand or on
an
>automatic slide stainer. Any histological technique book will tell you
that
>the deparaffinization is accomplished by using xylene, xylene
substitute or
>other appropriate paraffin solvent. Ideally, the slides should be
dried at
>room temperature overnight especially if photographs are going to be
made.
>But few labs have this much time. A drier that has a temperature in
excess
>of 60 degrees C will cause some artifacts. Our autostainer has a
built-in
>forced air dryer that the slides stay in for 10 - 15 minutes. If we
have a
>rack on the stainer and have another rack ready for the stainer, we
place
>this rack in a small forced air dryer set for 15 minutes then remove
and
>wait on the stainer to finish before adding another rack. The slide
dryer
>then is by-passed and the dried slides go directly to the first
xylene
>substitute (what we use instead of xylene) for deparaffinization. If
they
>still won't believe you, have them try to stain some "dried slides"
and see
>the results.
>
>Good Luck,
>Diane
>
>Diane C. Gladney, HT (ASCP)
>Histology /Cytology Supervisor
>Moncrief Army Community Hospital
>P.O. BOX 484
>4500 Stuart Ave.
>FT. Jackson, SC 29207
>
>(803) 751-2530
>DSN 734-2530
>
>EMAIL: diane.gladney <@t> se.amedd.army.mil  OR
>                  dcgx1 <@t> aol.com 
>
>
>-----Original Message-----
>From: Jordan Brod [mailto:jbrod <@t> tvmdl.tamu.edu] 
>Sent: Thursday, September 18, 2003 10:41 AM
>To: histonet <@t> lists.utsouthwestern.edu 
>Subject: [Histonet] Purpose of Oven Prior to Staining?
>
>
>Good Morning All:
>
>My lab is in a huge discussion about the reason why we put our slides
in an
>oven before staining.  I told them that the reason was to dry all of
the
>water off of them, because xylene and water do not mix.  This
statement is
>also backed up in literature that I have read.  They still do not
believe
>me, because they think the purpose of the oven is to deparaffinize
the
>slides.
>
>Can anyone help me out, so I can put this discussion to rest?  I will
print
>off all of your comments for my technicians to read.  Is what I told
them
>correct or not?
>
>Thanks.
>
>
>
>
>********************************************************************
>Jordan W. Brod
>Diagnostic Lab Supervisor - Pathology
>Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
>1 Sippel Road
>TAMU 4471
>College Station, Texas 77843
>(979) 845-3414
>(979) 845-1794 fax
>jbrod <@t> tvmdl.tamu.edu 
>
>
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Mr.Laurie Reilly                                              Ph 07
4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.


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