[Histonet] Fw: Brain Tissue in Buffer

[Geoff McAuliffe]

What exactly is the problem? If the tissue was properly fixed it should 
cut fine, no matter if storage was one week or one year (assuming no 
mold growth). Are you trying to embed in wax or cut frozen sections?

Geoff

Mary Parker wrote:

>  
> ----- Original Message -----
> From: Mary Parker <mailto:mparker <@t> epl-inc.com>
> To: histonet <@t> pathology.swmed.edu.com 
> <mailto:histonet <@t> pathology.swmed.edu.com>
> Sent: Monday, September 22, 2003 8:03 AM
> Subject: Brain Tissue in Buffer
>
> If anyone has any experience about how to successfully process and 
> microtome serial sections of rat brain tissue that was perfused, then 
> stored in buffer solution for approx one year in the 
> refrigerator...please help! We were given this tissue by an outside 
> investigator and are having a miserable time of it.
> I need all the information I can gather about tissue stored in buffer 
> for an extended period of time.
> Is it hopeless?
> Thanks
>  
>  
> Mary Parker, H.T., A.S.C.P.
> Histology Manager
> EPL, Inc.
> P.O. Box 12766
> RTP, N.C. 27709
> (919) 998-9407


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************


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