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What exactly is the problem? If the tissue was properly fixed it should
cut fine, no matter if storage was one week or one year (assuming no
mold growth). Are you trying to embed in wax or cut frozen sections?<br>
<br>
Geoff<br>
<br>
Mary Parker wrote:<br>
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style="font-family: arial; font-style: normal; font-variant: normal; font-weight: normal; font-size: 10pt; line-height: normal; font-stretch: normal; font-size-adjust: none;">-----
Original Message -----
<div
style="background: rgb(228, 228, 228) none repeat scroll 0%; -moz-background-clip: initial; -moz-background-inline-policy: initial; -moz-background-origin: initial;"><b>From:</b>
<a title="mparker@epl-inc.com" href="mailto:mparker@epl-inc.com">Mary
Parker</a> </div>
<div><b>To:</b> <a title="histonet@pathology.swmed.edu.com"
href="mailto:histonet@pathology.swmed.edu.com">histonet@pathology.swmed.edu.com</a>
</div>
<div><b>Sent:</b> Monday, September 22, 2003 8:03 AM</div>
<div><b>Subject:</b> Brain Tissue in Buffer</div>
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<div><font face="Arial" size="2">If anyone has any experience about
how to successfully process and microtome serial sections of rat brain
tissue that was perfused, then stored in buffer solution for approx one
year in the refrigerator...please help! We were given this tissue by an
outside investigator and are having a miserable time of it.</font></div>
<div><font face="Arial" size="2">I need all the information I can
gather about tissue stored in buffer for an extended period of time.</font></div>
<div><font face="Arial" size="2">Is it hopeless?</font></div>
<div><font face="Arial" size="2">Thanks</font></div>
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<div><font face="Arial" size="2">Mary Parker, H.T., A.S.C.P.<br>
Histology Manager<br>
EPL, Inc.<br>
P.O. Box 12766<br>
RTP, N.C. 27709<br>
(919) 998-9407</font></div>
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<pre class="moz-signature" cols="72">--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
<a class="moz-txt-link-abbreviated" href="mailto:mcauliff@umdnj.edu">mcauliff@umdnj.edu</a>
**********************************************</pre>
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