[Histonet] AEC substrate for IHC

[John Kiernan]

The answer is WATER.
You must use an aqueous mounting medium 
to preserve the product of oxidation of
AEC. You must not dehydrate or clear
the preparations.
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
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Baowei Peng wrote:
> 
> hi, all histoneters,
> 
> We can use ethanol to dehydrate and xylene to clear slides if we used DAB as substrate in IHC. Since AEC is organically soluble, we can not use these reagents.
> I am wondering what reagents we should use and should we dehydrate and clear AEC substrate slides?