[Histonet] AEC substrate for IHC

[Baowei Peng]

hi, all histoneters,

We can use ethanol to dehydrate and xylene to clear slides if we used DAB as substrate in IHC. Since AEC is organically soluble, we can not use these reagents.
I am wondering what reagents we should use and should we dehydrate and clear AEC substrate slides?

Baowei Peng
Shanghai JiaoTong University,
Shanghai,
China,PRC


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