[Histonet] how to embed/section mesentery?

[Geoff McAuliffe]

    Assuming that the tissue is flat enough (from tension applied during 
fixation and processing) you can put cleared tissue on a slide and 
coverslip it. Of course, it was stained (which I forgot to mention, 
blush!) after fixation and before dehydration and clearing. We use such 
spreads to teach the histology of loose connective tissue to first year 
medical students. Probably not something a clinical lab would do.

Geoff

Donna Kumiski wrote:

>How do you mount the tissue on the slide?
>    Donna
>
>----------------------------------------------
>Donna Kumiski     HT (ASCP)
>Medical College of Georgia
>Dept. of Cellular Biology and Anatomy
>CB 1202
>Augusta, Georgia   30912-2000   
>(706) 721-6278
> dkumiski <@t> mail.mcg.edu
>-----------------------------------------------
>
>
>  
>
>>>>Geoff McAuliffe <mcauliff <@t> umdnj.edu> 9/18/2003 10:55:23 AM >>>
>>>>        
>>>>
>Hi Felix:
>
>    For mesentaries some people like a "spread" rather than a section. 
>Take out several loops of intestine and pin them out on a sheet of cork 
>or dental wax with a tiny bit of tension on the mesentary so it is flat. 
>Submerge in fix. You can process the whole "block" this way throught 
>alcohols and clearing, then cut out and mount the sheet of mesentary on 
>a slide without embedding. Yes it will be thicker than a section but you 
>will see a nice 3D tissue with capillaries etc.
>
>Geoff
>
>Felix.Rintelen <@t> serono.com wrote:
>
>  
>
>>Dear all,
>>
>>Is there anybody who may give me advice how to make good sections of
>>parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
>>and how to handle it to embed/orient it properly in the parafin block? My
>>concern is especially the soft consistency of the tissue making me feel
>>that the proper orientation in the block might be difficult.
>>
>>Thank you very much in advance,
>>
>>Felix
>>
>>Felix Rintelen
>>Serono Pharmaceutical Research Institute
>>Geneva, Switzerland
>>
>>
>>
>>
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-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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