<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
<meta http-equiv="Content-Type" content="text/html;charset=ISO-8859-1">
<title></title>
</head>
<body text="#000000" bgcolor="#ffffff">
Assuming that the tissue is flat enough (from tension applied
during fixation and processing) you can put cleared tissue on a slide
and coverslip it. Of course, it was stained (which I forgot to mention,
blush!) after fixation and before dehydration and clearing. We use such
spreads to teach the histology of loose connective tissue to first year
medical students. Probably not something a clinical lab would do.<br>
<br>
Geoff<br>
<br>
Donna Kumiski wrote:<br>
<blockquote type="cite" cite="midsf6abdab.078@mail.mcg.edu">
<pre wrap="">How do you mount the tissue on the slide?
Donna
----------------------------------------------
Donna Kumiski HT (ASCP)
Medical College of Georgia
Dept. of Cellular Biology and Anatomy
CB 1202
Augusta, Georgia 30912-2000
(706) 721-6278
<a class="moz-txt-link-abbreviated" href="mailto:dkumiski@mail.mcg.edu">dkumiski@mail.mcg.edu</a>
-----------------------------------------------
</pre>
<blockquote type="cite">
<blockquote type="cite">
<blockquote type="cite">
<pre wrap="">Geoff McAuliffe <a class="moz-txt-link-rfc2396E" href="mailto:mcauliff@umdnj.edu"><mcauliff@umdnj.edu></a> 9/18/2003 10:55:23 AM >>>
</pre>
</blockquote>
</blockquote>
</blockquote>
<pre wrap=""><!---->Hi Felix:
For mesentaries some people like a "spread" rather than a section.
Take out several loops of intestine and pin them out on a sheet of cork
or dental wax with a tiny bit of tension on the mesentary so it is flat.
Submerge in fix. You can process the whole "block" this way throught
alcohols and clearing, then cut out and mount the sheet of mesentary on
a slide without embedding. Yes it will be thicker than a section but you
will see a nice 3D tissue with capillaries etc.
Geoff
<a class="moz-txt-link-abbreviated" href="mailto:Felix.Rintelen@serono.com">Felix.Rintelen@serono.com</a> wrote:
</pre>
<blockquote type="cite">
<pre wrap="">Dear all,
Is there anybody who may give me advice how to make good sections of
parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
and how to handle it to embed/orient it properly in the parafin block? My
concern is especially the soft consistency of the tissue making me feel
that the proper orientation in the block might be difficult.
Thank you very much in advance,
Felix
Felix Rintelen
Serono Pharmaceutical Research Institute
Geneva, Switzerland
********************************************************************************************
S - This message contains confidential information and is intended only for the individual
named. If you are not the named addressee, you should not disseminate, distribute or copy
this e-mail. Please notify the sender immediately by e-mail if you have received this
e-mail by mistake and delete this e-mail from your system.
e-mail transmission cannot be guaranteed to be secure or error-free as information could be
intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain malware. The
presence of this disclaimer is not a proof that it was originated at Serono International S.A.
or one of its affiliates. Serono International S.A and its affiliates therefore do not accept
liability for any errors or omissions in the content of this message, which arise as a result
of e-mail transmission. If verification is required, please request a hard-copy version.
Serono International SA, 15bis Chemin Des Mines, Geneva, Switzerland, <a class="moz-txt-link-abbreviated" href="http://www.serono.com">www.serono.com</a>.
*********************************************************************************************
_______________________________________________
Histonet mailing list
<a class="moz-txt-link-abbreviated" href="mailto:Histonet@lists.utsouthwestern.edu">Histonet@lists.utsouthwestern.edu</a>
<a class="moz-txt-link-freetext" href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</a>
</pre>
</blockquote>
<pre wrap=""><!---->
</pre>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
<a class="moz-txt-link-abbreviated" href="mailto:mcauliff@umdnj.edu">mcauliff@umdnj.edu</a>
**********************************************</pre>
</body>
</html>