[Histonet] Alizarin red/Alcian blue double skeletal stain

[John Kiernan]

There's this method:

Webb GN & Byrd RA 1994. simultaneous differential
staining of cartilage and bone in rodent fetuses: an
alcian blue and alizarin red S procedure without
glacial acetic acid. Biotechnic & Histochemistry
69(4):181-185. 

The colour photo looks excellent. The instructions take
up 2 printed pages so more is needed than a simple
"protocol." The journal is available in all good
medical libraries. The method could probably be
simplified for chick embryos because they are smaller
than rat fetuses.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
_______________________________________
"Grant, Debra" wrote:
> 
> Does anyone have a protocol for Alizarin Red/Alcian Blue on chick embryo? I have several protocols for mouse. I have tried to adjust times in solution, but I have some problems with the cartilage staining in some of the chick embryos.
> Thank you in advance.
> 
> Debby Grant
> Research Technician II
> Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th Street
> Kansas City, MO 64110
> drg <@t> stowers-institute.org