[Histonet] Re: Histonet digest, Vol 1 #53 - 32 msgs

[amosbrooks <@t> earthlink.net]

Jordan,

   You are right they are wrong. I agree with what Geoff said about having them stain an un-
deparaffinized section. He makes a good point.
   When you pick up a section there is a tiny film of water between the section and the slide. If 
you don't remove the film of water by drying the slide, the water will try to pull off the slide and 
mix with whatever solution it is in. (Regaurdless of using charged slides or not) That would be 
bad because the water pulling away from the slide would pull the section off with it. Like a 
sandwich and pulling the jelly out thru the bread. That is the reason for drying the slide.
   Another experiment to try. Don't dry some extra slides. Just run them down to water. Most of 
the tissue will have fallen off. That is just to the water water, then try staining them, especially 
with any harsh chemicals (peroxide, silver etc), there will be no tissue left.

It's nice being right isn't it?
later,
Amos Brooks


> Message: 14
> Date: Thu, 18 Sep 2003 09:41:22 -0500
> From: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Purpose of Oven Prior to Staining?
> 
> Good Morning All:
> 
> My lab is in a huge discussion about the reason why we put our slides
> in = an oven before staining.  I told them that the reason was to dry
> all of = the water off of them, because xylene and water do not mix. 
> This = statement is also backed up in literature that I have read. 
> They still do = not believe me, because they think the purpose of the
> oven is to deparaffin= ize the slides.
> 
> Can anyone help me out, so I can put this discussion to rest?  I will
> = print off all of your comments for my technicians to read.  Is what
> I told = them correct or not?
> 
> Thanks.