[Histonet] under processed tissue

[Atoska S. Gentry]

Hello, my processor recently malfunctioned rendering poorly processed 
sections. I placed the sections in the 60C incubator for 30 min. to aid in 
melting of the paraffin. Then I closely monitored the samples as I sent 
them backwards through the processing cycle. I actually reversed the 
beakers ( ie. placed #10 at station 1 and beaker #1 at station 10 and so on 
and so forth). If you decide to try this be sure to remember to switch them 
back to their proper stations and replace all solutions. Also, don't allow 
them to go past #10 ( avoiding the paraffin infiltration). I Washed 
sections for 30 min. in running tap H2O. Then placed them in a rehydrating 
solution: 0.6g sodium carbonate+ 42ml DHOH +18ml absolute alcohol 
overnight. Although, this method advises processing the following day on 
the standard 16-hour procedure, starting in 80% alcohol. I usually 
processed as usual starting in 70% ETOH. This rehydrating solution method 
was taken from Sheehan/Mosby Theory and Practice of Histotechnology  pge 4. 
My most recent samples I'm currently holding in 70% ETOH until I can get my 
processor problem resolved. However, I've used this method before and it 
renders fairly decent sections with a bit of trial and error. Best wishes. 
Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 
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