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Hello, my processor recently malfunctioned rendering poorly processed
sections. I placed the sections in the 60C incubator for 30 min. to aid
in melting of the paraffin. Then I closely monitored the samples as I
sent them backwards through the processing cycle. I actually reversed the
beakers ( ie. placed #10 at station 1 and beaker #1 at station 10 and so
on and so forth). If you decide to try this be sure to remember to switch
them back to their proper stations and replace all solutions. Also, don't
allow them to go past #10 ( avoiding the paraffin infiltration). I Washed
sections for 30 min. in running tap H2O. Then placed them in a
rehydrating solution: 0.6g sodium carbonate+ 42ml DHOH +18ml absolute
alcohol overnight. Although, this method advises processing the following
day on the standard 16-hour procedure, starting in 80% alcohol. I usually
processed as usual starting in 70% ETOH. This rehydrating solution method
was taken from Sheehan/Mosby <u>Theory and Practice of Histotechnology
</u> pge 4. My most recent samples I'm currently holding in 70% ETOH
until I can get my processor problem resolved. However, I've used this
method before and it renders fairly decent sections with a bit of trial
and error. Best wishes. Atoska<br><br>
<x-sigsep><p></x-sigsep>
Atoska S. Gentry B.S., HT(ASCP)<br>
Research Assistant III<br>
Scott-Ritchey Research Center<br>
College of Veterinary Medicine<br>
Auburn University, AL 36849<br>
Phone# (334)844-5579 Fax# (334)844-5850</body>
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