Fw: [Histonet] Purpose of Oven Prior to Staining?

[Gudrun Lang]

----- Original Message -----
From: "Gudrun Lang" <g.lang <@t> bigfoot.de>
To: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
Sent: Thursday, September 18, 2003 6:00 PM
Subject: Re: [Histonet] Purpose of Oven Prior to Staining?


> I think the purpose is to melt the paraffin, because warm liquid paraffin
is
> easier to get off the tissue in xylene.
> The second point is, that the tissue will be baked on the glass. So it
hold
> better.
> When you put the slides to early out of the oven, the cuts will swim off
the
> slide.
> Gundi Lang
> general hospital, Linz, Austria
>
>
> ----- Original Message -----
> From: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 18, 2003 4:41 PM
> Subject: [Histonet] Purpose of Oven Prior to Staining?
>
>
> Good Morning All:
>
> My lab is in a huge discussion about the reason why we put our slides in
an
> oven before staining.  I told them that the reason was to dry all of the
> water off of them, because xylene and water do not mix.  This statement is
> also backed up in literature that I have read.  They still do not believe
> me, because they think the purpose of the oven is to deparaffinize the
> slides.
>
> Can anyone help me out, so I can put this discussion to rest?  I will
print
> off all of your comments for my technicians to read.  Is what I told them
> correct or not?
>
> Thanks.
>
>
>
>
> ********************************************************************
> Jordan W. Brod
> Diagnostic Lab Supervisor - Pathology
> Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
> 1 Sippel Road
> TAMU 4471
> College Station, Texas 77843
> (979) 845-3414
> (979) 845-1794 fax
> jbrod <@t> tvmdl.tamu.edu
>
>
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