[Histonet] Colloidal Iron

[Smith, Allen]

The original Hale's colloidal iron is considered unreliable (Pearse,
HISTOCHEMISTRY, 3rd ed. 1968; Zugibe, DIAGNOSTIC HISTOCHEMISTRY, 1970).
Several more reliable modifications have appeared.  Mowry's (1958, J. Clin.
Invest. 7:566-576) is the easiest to use, but is erratic in my hands.
Rinehart and Abul-Haj's method (1951, Arch. Pathol. 52:189-194) is terribly
time-consuming but consistent. I have used both  Mowry's method and Rinehart
and Abul-Haj's method on prostate carcinoma, extramammary Paget's disease,
Barrett's esophagus, and the sialomucins of the intercellular canaliculi of
eccrine sweat glands.

-----Original Message-----
From: lena spencer [mailto:lenaspencer <@t> insightbb.com] 
Sent: Monday, September 15, 2003 8:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Colloidal Iron


Hi All:
One of my Pathologist's has requested a Hale's Colloidal Iron for renal cell
carcinoma chromophobes.  I am familiar with colloidal iron for
mucosubstances, but he feel's that this is not the same stain.   The article
stated that the diagnosis is made from the colloidal iron stain  or from
electron microscopy .  This Pathologist is requesting that I post on the
histonet for additional information or for someone out in histoland who is
performing this procedure. Your is insight would be greatly appreciated.
Lena



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