[Histonet] questions regarding to a-Naphthyl ButyrateEsterase stain and a-Naphthyl Acetate stain(no

[Pamela Marcum]

and filter paper requirements should be followed use exactly what they say
or risk the reaction hydrolyzing in the filter.  Pam Marcum

> -----Original Message-----
> From: histonet-admin <@t> lists.utsouthwestern.edu
> [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Fred
> Underwood
> Sent: Wednesday, September 17, 2003 10:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] questions regarding to a-Naphthyl
> ButyrateEsterase stain and a-Naphthyl Acetate stain(no
>
>
> I have used the kits put together by  Sigma.  The results were good.
> However, strict adherence to expiration dates, temperatures, times and
> solution prepatation is necessary.  Otherwise the results can be
> variable and less than optimal.
>
> Fred
>
> >>> weiping Ren <weipingren <@t> yahoo.com> 09/16/03 01:13PM >>>
> Hi, Histonetters:
> I need to set up non specific esterase staining method on bone marrow
> smear samples.
>
> My questions are:
> 1. Are a-Naphthyl Butyrate Esterase stain and a-Naphthyl Acetate
> stain(non specific esterase) are the same stain?
> 2. If these are two different stains but have the same clinical
> diagnosis significance, which one is better regarding to the time and
> accuracy?
> 3. Are there any protocols for these stains? Where I can get these
> references?
>
> Thank you!
>
> Any suggestions and advice welcome.
>
> Weiping
> Wayne State University
> Detroit, MI
>
>
>
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