[Histonet] questions regarding to a-Naphthyl Butyrate Esterase stain and a-Naphthyl Acetate stain(no

[Fred Underwood]

I have used the kits put together by  Sigma.  The results were good. 
However, strict adherence to expiration dates, temperatures, times and
solution prepatation is necessary.  Otherwise the results can be
variable and less than optimal.

Fred

>>> weiping Ren <weipingren <@t> yahoo.com> 09/16/03 01:13PM >>>
Hi, Histonetters:
I need to set up non specific esterase staining method on bone marrow
smear samples. 
 
My questions are:
1. Are a-Naphthyl Butyrate Esterase stain and a-Naphthyl Acetate
stain(non specific esterase) are the same stain?
2. If these are two different stains but have the same clinical
diagnosis significance, which one is better regarding to the time and
accuracy?
3. Are there any protocols for these stains? Where I can get these
references?
 
Thank you!
 
Any suggestions and advice welcome.
 
Weiping 
Wayne State University
Detroit, MI 



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