[Histonet] haematoxylin

[Davis, Gareth]

Thanks for all the responses to the Hematoxylin problem.  I'm going to Ehrlich's and see how that goes.
Gareth
 
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of 
the Department of Neurology
Vanderbilt University Medical Center
615-936-3318
 
 

	-----Original Message----- 
	From: damien desmond [mailto:vanlochemstraat <@t> yahoo.com] 
	Sent: Fri 9/12/2003 7:04 AM 
	To: Davis, Gareth 
	Cc: 
	Subject: RE: [Histonet] haematoxylin
	
	

	Harris and Weigert are regressive stains, so the
	stains overstain and then you decolourize to the
	desired effect, Mayers is progressive so no use of a
	differentiator and this will stain to an end point in
	a certain time period to be determined by testing and
	microscopic interpretation.
	
	David
	--- "Davis, Gareth" <gareth.davis <@t> Vanderbilt.Edu>
	wrote:
	> I'm trying to find a good Hematoxylin for good H&E
	> results, is that what your discussion was about?
	> Gill's is best for counterstaining IHC sections, in
	> my opinion.
	> Gareth
	> 
	> Ms. Gareth B. Davis
	> Research Assistant II
	> Neuro-magnetics Division of
	> the Department of Neurology
	> Vanderbilt University Medical Center
	> 615-936-3318
	> 
	> 
	>
	>       -----Original Message-----
	>       From: Gudrun Lang [mailto:g.lang <@t> bigfoot.de]
	>       Sent: Thu 9/11/2003 12:44 PM
	>       To: Histonetliste
	>       Cc:
	>       Subject: [Histonet] haematoxylin
	>      
	>      
	>       In our lab we have the discussion, if staining with
	> Mayer's or Harris' or Weigert's hematoxylin leads to
	> an endpoint-staining.
	>       I have read, that only Weigert stains to an
	> endpoint. The others can be overstained.
	>       Who can explain and help?
	>       
	>       Gundi
	>       Akh, Linz, Austria
	>
	>
	
	
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