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<TITLE>RE: [Histonet] haematoxylin</TITLE>
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<DIV>Thanks for all the responses to the Hematoxylin problem. I'm going to
Ehrlich's and see how that goes.</DIV>
<DIV>Gareth</DIV>
<DIV> </DIV>
<DIV><FONT face=System color=#800080 size=2>Ms. Gareth B. Davis</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2><STRONG>Research Assistant
II</STRONG></FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Neuro-magnetics Division of
</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>the Department of
Neurology</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>Vanderbilt University Medical
Center</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2>615-936-3318</FONT></DIV>
<DIV><FONT face=System color=#800080 size=2></FONT> </DIV>
<DIV> </DIV>
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<DIV><FONT size=2>-----Original Message----- <BR><B>From:</B> damien desmond
[mailto:vanlochemstraat@yahoo.com] <BR><B>Sent:</B> Fri 9/12/2003 7:04 AM
<BR><B>To:</B> Davis, Gareth <BR><B>Cc:</B> <BR><B>Subject:</B> RE: [Histonet]
haematoxylin<BR><BR></FONT></DIV>
<P><FONT size=2>Harris and Weigert are regressive stains, so the<BR>stains
overstain and then you decolourize to the<BR>desired effect, Mayers is
progressive so no use of a<BR>differentiator and this will stain to an end
point in<BR>a certain time period to be determined by testing
and<BR>microscopic interpretation.<BR><BR>David<BR>--- "Davis, Gareth"
<gareth.davis@Vanderbilt.Edu><BR>wrote:<BR>> I'm trying to find a
good Hematoxylin for good H&E<BR>> results, is that what your
discussion was about?<BR>> Gill's is best for counterstaining IHC sections,
in<BR>> my opinion.<BR>> Gareth<BR>> <BR>> Ms. Gareth B.
Davis<BR>> Research Assistant II<BR>> Neuro-magnetics Division
of<BR>> the Department of Neurology<BR>> Vanderbilt University Medical
Center<BR>> 615-936-3318<BR>> <BR>> <BR>><BR>>
-----Original Message-----<BR>>
From: Gudrun Lang [<A
href="mailto:g.lang@bigfoot.de">mailto:g.lang@bigfoot.de</A>]<BR>>
Sent: Thu 9/11/2003 12:44 PM<BR>>
To: Histonetliste<BR>>
Cc:<BR>>
Subject: [Histonet] haematoxylin<BR>>
<BR>> <BR>>
In our lab we have the discussion, if staining
with<BR>> Mayer's or Harris' or Weigert's hematoxylin leads to<BR>> an
endpoint-staining.<BR>> I have read, that
only Weigert stains to an<BR>> endpoint. The others can be
overstained.<BR>> Who can explain and
help?<BR>> <BR>>
Gundi<BR>>
Akh, Linz,
Austria<BR>><BR>><BR><BR><BR>__________________________________<BR>Do
you Yahoo!?<BR>Yahoo! SiteBuilder - Free, easy-to-use web site design
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href="http://sitebuilder.yahoo.com">http://sitebuilder.yahoo.com</A><BR></FONT></P></BLOCKQUOTE>
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