[Histonet] SFOG

[John Kiernan]

pH between 1 and 2 should be about right for a
trichrome.
The acidity is due to the phosphotungstic or
phosphomolybdic acid, which is an essential ingredient.
Checking the pH is not part of the routine of making
up these solutions.

It is important to rinse in acidified water after 
staining, and I advise taking the slides directly
from the acidified water to the first of 3 lots of
100% alcohol. The dyes are easily extracted from
the sections by non-acidified water or by
water-alcohol mixtures.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
___________________________
> Gudrun Lang wrote:
> 
> Hi!
> Do you know, what pH the dye-solution of SFOG has to have?
> SFOG (= Säurefuchsin-Orange-G-Anilinblau) is similar to Cason's onestep trichrome staining solution.
> It must be something about 1,09 - 1,9.
> 
> thanks, Gudrun