[Histonet] nuclear counterstain

[kgibbon <@t> qltinc.com]

I have used Vectors VIP kit and found it excellent. I have used both
Nuclear fast red and Mayer's haematoxylin for a counterstain and find that
the blue of the Mayer's is best for my work. That includes image analysis.

Kevin Gibbon
QLT Inc.


                                                                                                                                                 
                      "Atoska S. Gentry"                                                                                                         
                      <gentras <@t> vetmed.auburn.edu>         To:       Histonet <histonet <@t> pathology.swmed.edu>                                      
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                      histonet-admin <@t> lists.utsouth        Subject:  [Histonet] nuclear counterstain                                              
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                      09/12/03 08:15 AM                                                                                                          
                                                                                                                                                 
                                                                                                                                                 





Hello again, guess I need to rephrase my initial inquiry on the
counterstain to use with the Vectastain ABC-HRP kit. But, first special
thanks to Dr. John Kiernan. He always replies with such informative and
thorough remarks. Please pardon my partial entry of facts. This inquiry is
on behalf of a couple of colleagues of mine. And yes their work was with
monolayers of cultured bone marrow cells. However, the HRP was detected
with the Vector VIP substrate       ( for which Vector Labs does not reveal

the contents) and yielded neurons that are purple in color. Therefore, we
need something like acridine orange which stains DNA. Has anyone had any
experience/success using acridine orange or something similar with this
system that yields the same results? Thanks, Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850


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