[Histonet] effects of decalcifying reagents on probes

[Nava, Josefa]

Hello to all,

Does anybody know  if decalcifying reagents affect the tissue(bone) when in
situ  (kappa, lambda, RNA+) is to be done on the tissue?
I did a kappa ,lambda ,RNA+ on a decaled bone and I have no staining on all
of them, but the controls are good.  Any suggestions ?
I thank you very much. By the way I am using the Ventana in situ reagents,
and have talked to their tech support but did not get good explanation .


Josie


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