[Histonet] Orcein Variation for EVG to detect Elastosis Perfo rans Serpiginosum

[Tony Henwood]

Kevin,
 
The following article may be of use:
 
Henwood, A., (2002) "The Orcein Stain - A Versatile Stain for
Histopathology" J. Histotechnol 25(1):29.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager 
The Children's Hospital at  Westmead, 
Locked Bag 4001, Westmead, 2145, AUSTRALIA. 
Tel: 612 9845 3306 
Fax: 612 9845 3318 

http://www.histosearch.com/homepages/TonyHenwood/default.html
<http://www.histosearch.com/homepages/TonyHenwood/default.html>  
http://us.geocities.com/tonyhenwoodau/index.html
<http://us.geocities.com/tonyhenwoodau/index.html>  

-----Original Message-----
From: kevin williams [mailto:akwilliams75 <@t> hotmail.com]
Sent: Monday, 1 September 2003 4:15
To: MTitford <@t> aol.com; histonet <@t> pathology.swmed.edu
Subject: [Histonet] Orcein Variation for EVG to detect Elastosis Perforans
Serpiginosum











Dear MT( and all you HTs out there):

Thanks for the information lots of help:

I am now on the quest for a procedure for Elastosis Perforans Serpiginosum.

My pathologist has asked for the Orcein variation on the EVG(Elastin Van
Gieson) or VVG as some of you know it. I have to get the Orcein so that is
my first challenge, second is my protocol. There are a few out there and I
would like to here from anyone who has a favorite for this method with this
disease process. 

Yours faithfully A. Keivn Williams



>From: MTitford <@t> aol.com 
>To: Histonet <@t> lists.utsouthwestern.edu 
>Subject: [Histonet] Alpha Amylase 
>Date: Fri, 29 Aug 2003 15:50:51 -0400 
> 
>Robert Lotts asks about amylase: 
>We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees centigrade
for 30 minutes). I zap it in the microwave to get it up to temperature 
> 
>Mike Titford 
>USA Pathology 
>Mobile AL 36617 USA 
> 
>_______________________________________________ 
>Histonet mailing list 
>Histonet <@t> lists.utsouthwestern.edu 
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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