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<DIV><SPAN class=480475703-01092003><FONT face=Arial color=#0000ff
size=2>Kevin,</FONT></SPAN></DIV>
<DIV><SPAN class=480475703-01092003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=480475703-01092003><FONT face=Arial color=#0000ff size=2>The
following article may be of use:</FONT></SPAN></DIV>
<DIV><SPAN class=480475703-01092003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=480475703-01092003><FONT face=Arial color=#0000ff
size=2>Henwood, A., (2002) "The Orcein Stain - A Versatile Stain for
Histopathology" J. Histotechnol 25(1):29.</DIV>
<P><FONT face=Arial size=2>Tony Henwood JP, B</FONT><FONT face=Arial size=2>A</FONT><FONT face=Arial size=2>ppSc, GradDipSysAnalys, CT(ASC)</FONT>
<BR><FONT face=Arial size=2>Laboratory Manager</FONT> <BR><FONT face=Arial
size=2>The Children's Hospital at Westmead,</FONT> <BR><FONT face=Arial
size=2>Locked Bag 4001, Westmead, 2145, AUSTRALIA.</FONT> <BR><FONT face=Arial
size=2>Tel:</FONT> <FONT face=Arial size=2>61</FONT><FONT face=Arial size=2>2
9845 3306</FONT> <BR><FONT face=Arial size=2>Fax:</FONT> <FONT face=Arial
size=2>61</FONT><FONT face=Arial size=2>2 9845 3318</FONT> </P>
<P><FONT face=Arial size=2><A target=_blank
href="http://www.histosearch.com/homepages/TonyHenwood/default.html">http://www.histosearch.com/homepages/TonyHenwood/default.html</A></FONT>
<BR><FONT face=Arial size=2><A target=_blank
href="http://us.geocities.com/tonyhenwoodau/index.html">http://us.geocities.com/tonyhenwoodau/index.html</A></FONT>
</P></FONT></SPAN>
<BLOCKQUOTE>
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> kevin williams
[mailto:akwilliams75@hotmail.com]<BR><B>Sent:</B> Monday, 1 September 2003
4:15<BR><B>To:</B> MTitford@aol.com;
histonet@pathology.swmed.edu<BR><B>Subject:</B> [Histonet] Orcein Variation
for EVG to detect Elastosis Perforans Serpiginosum<BR><BR></FONT></DIV>
<DIV>
<DIV>
<P><BR><BR></P>
<DIV>
<DIV></DIV>
<P><BR><BR></P></DIV>
<DIV></DIV>
<DIV></DIV></DIV>
<P>Dear MT( and all you HTs out there):</P>
<DIV></DIV>
<DIV></DIV>
<P>Thanks for the information lots of help:</P>
<DIV></DIV>
<DIV></DIV>
<P>I am now on the quest for a procedure for Elastosis Perforans
Serpiginosum.</P>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<P>My pathologist has asked for the Orcein variation on the EVG(Elastin Van
Gieson) or VVG as some of you know it. I have to get the Orcein so that is my
first challenge, second is my protocol. There are a few out there and I would
like to here from anyone who has a favorite for this method with this disease
process. </P>
<P>Yours faithfully A. Keivn Williams<BR><BR></P>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>From: MTitford@aol.com
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>To: Histonet@lists.utsouthwestern.edu
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Subject: [Histonet] Alpha Amylase
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Date: Fri, 29 Aug 2003 15:50:51 -0400
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Robert Lotts asks about amylase:
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees
centigrade for 30 minutes). I zap it in the microwave to get it up to
temperature
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Mike Titford
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>USA Pathology
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Mobile AL 36617 USA
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>_______________________________________________
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Histonet mailing list
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>Histonet@lists.utsouthwestern.edu
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet <DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV>
<DIV></DIV></DIV><BR clear=all>
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