[Histonet] Sirius red

John Kiernan jkiernan <@t> uwo.ca
Fri Oct 31 10:58:22 CST 2003


CI 35780 is sirius red F3B (= Direct red 80).
It should work in a picro-sirius red method.
If it really fails, please let us all know 
the details - supplier, batch etc.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
______________________________

Andrea Grantham wrote:
> 
> I have a quick question about Sirius red since it is a recent topic of
> discussion on histonet.
> I was asked to do the stain and given a protocol calling for Sirius red F3B
> (CI 35782). I have Sirius red CI 35780. They said that they have tried the
> stain using CI 35780 and it didn't work but they cannot find the other
> Sirius red with a CI# 35782 in any of the catalogs. We have tried Sigma,
> Polysciences, PolyScientific, Newcomer, and some others...
> Any suggestions where I can find it?
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
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> : (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
> :...................................................................:
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