[Histonet] freezing bones

[Garry Ashton]

Dear all,
After fixing and decalcifying some mouse bones, does anybody have
experience at the best way to freeze the tissue down.
Is simply a sucrose gradient, followed by snap freezing in liquid N2
adequate?
Any advice and tips greatly appreciated.
 
Garry Ashton
 
 
Histology Dept
PICR
UK
 
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