<HTML xmlns:eXclaimer="http://www.exclaimer.co.uk">
<HEAD>
<META http-equiv="Content-Type" content="text/html; charset=UTF-16">
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=UTF-16"><TITLE>Message</TITLE>
<META http-equiv=Content-Type content="text/html; charset=us-ascii">
<META content="MSHTML 6.00.2600.0" name=GENERATOR></HEAD><BODY ><DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>Dear
all,</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>After fixing and
decalcifying some mouse bones, does anybody have experience at the best way to
freeze the tissue down.</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>Is simply a sucrose
gradient, followed by snap freezing in liquid N2 adequate?</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>Any advice and tips
greatly appreciated.</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=829342016-29102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>Garry
Ashton</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=829342016-29102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN
class=829342016-29102003></SPAN></FONT> </DIV>
<DIV><FONT face=Arial size=2><SPAN class=829342016-29102003>Histology
Dept</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=829342016-29102003>PICR</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN
class=829342016-29102003>UK</SPAN></FONT></DIV></DIV>
<DIV>
<HR>
</DIV>
<DIV ALIGN="left">
<FONT SIZE="2">This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.</FONT>
</DIV>
<FONT>
</FONT></BODY></HTML>