[Histonet] Prefer formalin-free fixative

[Gehan, Loralee]

Hi Amy, 
We just ordered some of this stuff and we haven't worked out all of the
conditions to make sure that the fixation is complete.  BUT,  we have fixed
mouse hind limbs and tail.  Some for 6 hours and some for overnight.  I
think that due to the size of the tissue that the over night is better.
The best part of it all, it doesn't smell like formalin.  
I haven't had a chance to try any IHC on these samples but the company
claims that you need to do little if any antigen retrieval.  Our special
staining has been beautiful.  Nuclear detail is great. 
Hope that helps. 

Loralee Gehan
Orthopaedics Research Lab
University of Rochester

> ----------
> From: 	Amy Self
> Sent: 	Wednesday, October 29, 2003 6:23 AM
> To: 	'histonet <@t> lists.utsouthwestern.edu'
> Subject: 	[Histonet] Prefer formalin-free fixative
> 
> 
> 
> 	Dear Histonetters,
> 
> 
> 	Has anyone tried the Prefer formalin-free fixative distributed by
> Anatech LTD.? If so, what were you likes and dislikes about it.
> Thanks in advance for your responses.
> 
> 
> 	Amy Self
> 	Georgetown Hospital
> 	
> 
> 
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