[Histonet] LMD ... IHC sections falling off

[Deanne Catmull]

Dear Victoria,

We always use Vectabond treated slides for immunohistochemistry. Sections
don't tend to float off. It's a simple procedure and good to use for pretty
much any stain. 

Deanne at La Trobe Bundoora
At 05:08 PM 10/23/03 -0500, mrl0627 <@t> mail.ecu.edu wrote:
>
> I sometimes lose sections (4 um thick, mounted on superfrost-plus) during
the counterstaining-clearing process although they survive immuno and
development in DAB.  Any thoughts of what I could be doing wrong?
>It's a heart breaker to lose them at the very end of the process.  I am in
a university lab and do everything by hand.  Thanks, Maureen at ECU
>-----Original Message-----
>From: Amos Brooks &lt;amosbrooks <@t> earthlink.net&gt;
>To: histonet <@t> lists.utsouthwestern.edu
>Sent: Mon, 20 Oct 2003 16:20:39 -0400
>Subject: [Histonet] LMD ... IHC sections falling off
>
>Victoria,
>         Firstly try thinner sections 10 um is a bit thick. Next you should 
>use some type of charged, silanized or APES slides. You can either silanize 
>your own slides or buy them from a vendor. Adhesives aren't necessarily 
>good for IHC as they can interfere with the reaction.
>Amos Brooks
>
>
>
>&gt;Message: 5
>&gt;From: victoria.hahn-stromberg <@t> orebroll.se
>&gt;To: histonet <@t> lists.utsouthwestern.edu
>&gt;Date: Mon, 20 Oct 2003 13:20:39 +0200
>&gt;Subject: [Histonet] LMD
>&gt;
>&gt;Hi!
>&gt;I=B4m having problems with my LMD slides.  I use 10um paraffin embedded
>&gt;histological sections which I would like to stain immunohistochemically =
>&gt;with
>&gt;CAM, but the sections keep falling off, can anyone plesse help =
>&gt;me??/Victoria
>&gt;
>&gt;Victoria Hahn-Str=F6mberg
>&gt;MSc, PhD student
>&gt;Kliniken f=F6r Patologi
>&gt;Universitetssjukhuset =D6rebro
>&gt;701 85 =D6rebro
>&gt;tel:  019-6023644
>&gt;faxnr:019-6021035
>
>
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Deanne Catmull	
Neuroimmunology Laboratory
Dept of Biochemistry
La Trobe University
Bundoora Vic 3086
Ph:9479-1155