[Histonet] LMD ... IHC sections falling off

[edmondsj]

Hello,
Try incubating your slides for a longer period before deparaffinizing that 
may help with the sections washing off.  Hope this helps.
Joyce Edmonds
MUSC
Charleston, SC

--On Thursday, October 23, 2003 5:08 PM -0500 mrl0627 <@t> mail.ecu.edu wrote:

>
>  I sometimes lose sections (4 um thick, mounted on superfrost-plus)
> during the counterstaining-clearing process although they survive immuno
> and development in DAB.  Any thoughts of what I could be doing wrong?
> It's a heart breaker to lose them at the very end of the process.  I am
> in a university lab and do everything by hand.  Thanks, Maureen at ECU
> -----Original Message-----
> From: Amos Brooks &lt;amosbrooks <@t> earthlink.net&gt;
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Mon, 20 Oct 2003 16:20:39 -0400
> Subject: [Histonet] LMD ... IHC sections falling off
>
> Victoria,
>          Firstly try thinner sections 10 um is a bit thick. Next you
> should  use some type of charged, silanized or APES slides. You can
> either silanize  your own slides or buy them from a vendor. Adhesives
> aren't necessarily  good for IHC as they can interfere with the reaction.
> Amos Brooks
>
>
>
> &gt;Message: 5
> &gt;From: victoria.hahn-stromberg <@t> orebroll.se
> &gt;To: histonet <@t> lists.utsouthwestern.edu
> &gt;Date: Mon, 20 Oct 2003 13:20:39 +0200
> &gt;Subject: [Histonet] LMD
> &gt;
> &gt;Hi!
> &gt;I=B4m having problems with my LMD slides.  I use 10um paraffin
> embedded &gt;histological sections which I would like to stain
> immunohistochemically = &gt;with
> &gt;CAM, but the sections keep falling off, can anyone plesse help =
> &gt;me??/Victoria
> &gt;
> &gt;Victoria Hahn-Str=F6mberg
> &gt;MSc, PhD student
> &gt;Kliniken f=F6r Patologi
> &gt;Universitetssjukhuset =D6rebro
> &gt;701 85 =D6rebro
> &gt;tel:  019-6023644
> &gt;faxnr:019-6021035
>
>
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