[Histonet] LMD ... IHC sections falling off
edmondsj
edmondsj <@t> musc.edu
Fri Oct 24 06:54:45 CDT 2003
Hello,
Try incubating your slides for a longer period before deparaffinizing that
may help with the sections washing off. Hope this helps.
Joyce Edmonds
MUSC
Charleston, SC
--On Thursday, October 23, 2003 5:08 PM -0500 mrl0627 <@t> mail.ecu.edu wrote:
>
> I sometimes lose sections (4 um thick, mounted on superfrost-plus)
> during the counterstaining-clearing process although they survive immuno
> and development in DAB. Any thoughts of what I could be doing wrong?
> It's a heart breaker to lose them at the very end of the process. I am
> in a university lab and do everything by hand. Thanks, Maureen at ECU
> -----Original Message-----
> From: Amos Brooks <amosbrooks <@t> earthlink.net>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Mon, 20 Oct 2003 16:20:39 -0400
> Subject: [Histonet] LMD ... IHC sections falling off
>
> Victoria,
> Firstly try thinner sections 10 um is a bit thick. Next you
> should use some type of charged, silanized or APES slides. You can
> either silanize your own slides or buy them from a vendor. Adhesives
> aren't necessarily good for IHC as they can interfere with the reaction.
> Amos Brooks
>
>
>
> >Message: 5
> >From: victoria.hahn-stromberg <@t> orebroll.se
> >To: histonet <@t> lists.utsouthwestern.edu
> >Date: Mon, 20 Oct 2003 13:20:39 +0200
> >Subject: [Histonet] LMD
> >
> >Hi!
> >I=B4m having problems with my LMD slides. I use 10um paraffin
> embedded >histological sections which I would like to stain
> immunohistochemically = >with
> >CAM, but the sections keep falling off, can anyone plesse help =
> >me??/Victoria
> >
> >Victoria Hahn-Str=F6mberg
> >MSc, PhD student
> >Kliniken f=F6r Patologi
> >Universitetssjukhuset =D6rebro
> >701 85 =D6rebro
> >tel: 019-6023644
> >faxnr:019-6021035
>
>
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