[Histonet] Embryo sticking in InsituPro Run

[Jimmy Lao]

Hi
Any one can help preventing embryo sticking to the
Column during InsituPro run? InsituPro is automated
machine for whole mount embryo in situ hybridization. 
It did not happen when I used fresh column. But it did
in the 2nd time using column.  I guess there are
something staying in the column after first use, even
I treated them with H2O2 overnight and 4%PFA cleaning
program treatment.  How can I clean used column?
Thanks!
Jimmy

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