[Histonet] Grocott's stain for fungi

[Bartlett, Jeanine]

And I do chemically clean the glassware used for the methenamine since it is
a component of the methenamine-silver.
 
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333

-----Original Message-----
From: Jackie.O'Connor <@t> abbott.com [mailto:Jackie.O'Connor <@t> abbott.com] 
Sent: Tuesday, October 28, 2003 12:02 PM
To: Kathleen Roberts
Cc: Histonet
Subject: Re: [Histonet] Grocott's stain for fungi



Make sure you are using the correct chemical, it should be
Hexamethylenetetramine (methenamine) (C6H12N4).  It should dissolve readily
in H20. 

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics




	Kathleen Roberts <kgrobert <@t> rci.rutgers.edu> 
Sent by: histonet-admin <@t> lists.utsouthwestern.edu 


10/28/2003 09:51 AM 


        
        To:        Histonet <histonet <@t> pathology.swmed.edu> 
        cc:         
        Subject:        [Histonet] Grocott's stain for fungi



Hi all-

I'm making up the various stock & working solutions for Grocott's stain, 
and I am having a heck of a time getting the 3% methenamine to go into 
solution.  (Not methenamine silver, just plain old 3% methenamine in 
distilled water.)  I know that you have to acid-clean the glassware for 
the methenamine silver & silver nitrate solutions, but do I have to do 
it for 3% methenamine?  I tried heating it, but that did not make a bit 
of difference. AFIP manual  has no other instructions other than "3 g 
methenamine in 100 ml distilled water".

Is there a shelf life for methenamine?  The bottle we have is pretty 
old, but I am not sure if that would be a factor.

I intend to try acid-cleaning a bottle for the 3% methenamine this 
afternoon, but I wanted to see if anybody had any other suggestions to 
try as well.

Thanks in advance-
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University



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