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<DIV><SPAN class=960242217-28102003><FONT face=Arial color=#0000ff size=2>And I
do chemically clean the glassware used for the methenamine since it is a
component of the methenamine-silver.</FONT></SPAN></DIV>
<DIV><SPAN class=960242217-28102003><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=960242217-28102003>
<DIV align=left><FONT face=Arial size=2>Jeanine Bartlett, HT(ASCP)</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Centers for Disease Control</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Infectious Disease Pathology
Activity</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>1600 Clifton Road, MS/G-32</FONT></DIV>
<DIV align=left><FONT face=Arial size=2>Atlanta, GA
30333</FONT></DIV></SPAN></DIV>
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<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com]
<BR><B>Sent:</B> Tuesday, October 28, 2003 12:02 PM<BR><B>To:</B> Kathleen
Roberts<BR><B>Cc:</B> Histonet<BR><B>Subject:</B> Re: [Histonet] Grocott's
stain for fungi<BR><BR></FONT></DIV><BR><FONT face=Arial size=2>Make sure you
are using the correct chemical, it should be Hexamethylenetetramine
(methenamine) (C6H12N4). It should dissolve readily in H20.</FONT>
<BR><BR><FONT face=Arial size=2>Jacqueline M. O'Connor HT(ASCP)<BR>Abbott
Laboratories<BR>Global Pharmaceutical Research and Development<BR>Discovery
Chemotheraputics<BR></FONT><BR><BR><BR>
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<TD><FONT face=sans-serif size=1><B>Kathleen Roberts
<kgrobert@rci.rutgers.edu></B></FONT> <BR><FONT face=sans-serif
size=1>Sent by: histonet-admin@lists.utsouthwestern.edu</FONT>
<P><FONT face=sans-serif size=1>10/28/2003 09:51 AM</FONT> <BR></P>
<TD><FONT face=Arial size=1> </FONT><BR><FONT
face=sans-serif size=1> To:
Histonet <histonet@pathology.swmed.edu></FONT>
<BR><FONT face=sans-serif size=1> cc:
</FONT> <BR><FONT face=sans-serif size=1>
Subject: [Histonet]
Grocott's stain for fungi</FONT></TR></TBODY></TABLE><BR><BR><BR><FONT
face="Courier New" size=2>Hi all-<BR><BR>I'm making up the various stock &
working solutions for Grocott's stain, <BR>and I am having a heck of a time
getting the 3% methenamine to go into <BR>solution. (Not methenamine
silver, just plain old 3% methenamine in <BR>distilled water.) I know
that you have to acid-clean the glassware for <BR>the methenamine silver &
silver nitrate solutions, but do I have to do <BR>it for 3% methenamine?
I tried heating it, but that did not make a bit <BR>of difference. AFIP
manual has no other instructions other than "3 g <BR>methenamine in 100
ml distilled water".<BR><BR>Is there a shelf life for methenamine? The
bottle we have is pretty <BR>old, but I am not sure if that would be a
factor.<BR><BR>I intend to try acid-cleaning a bottle for the 3% methenamine
this <BR>afternoon, but I wanted to see if anybody had any other suggestions
to <BR>try as well.<BR><BR>Thanks in advance-<BR>Kathleen Roberts<BR>Principal
Lab Technician<BR>Neurotoxicology Labs<BR>Dept of Pharmacology &
Toxicology<BR>Ernest Mario School of Pharmacy<BR>Rutgers
University<BR><BR><BR><BR>_______________________________________________<BR>Histonet
mailing
list<BR>Histonet@lists.utsouthwestern.edu<BR>http://lists.utsouthwestern.edu/mailman/listinfo/histonet<BR></FONT><BR><BR></BLOCKQUOTE></BODY></HTML>