[Histonet] RE: Digital Photomicroscopy

[K. Bowden]

I don't have a suggestion for a camera but I have heard that for 
publication quality photos you need to have a 6 to 7 megapixel camera.  
Then once you have solved that issue you need to have the proper distant 
from the camera to the scope and that is the real problem.  We are still 
working on that problem in our lab.

Karen Bowden
Staff Research Associate II
UCSD
La Jolla, CA

Gordon Grant wrote:

>Hello histonetters,
>I have an Olympus BH-2 microscope fitted with a real nice PM10AD Olympus
>film camera. I have been real happy with the set up as I take a lot of
>publication photos. My problem is digital is now the way to go. I
>borrowed a Coolpix MDC lens adapter with a coolpix and Olympus C-5050
>camera. I not very pleased with the results. Does anyone have a
>recommendation for me.
>
>Best
>Gordon Grant 
>
>-----Original Message-----
>From: histonet-admin <@t> lists.utsouthwestern.edu
>[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of
>histonet-request <@t> lists.utsouthwestern.edu
>Sent: Saturday, October 25, 2003 10:00 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Histonet digest, Vol 1 #101 - 12 msgs
>
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>
>Today's Topics:
>
>   1. Re: Histonet digest, Vol 1 #100 - 15 msgs (amosbrooks)
>   2. Re: CD 25 (Dana Settembre)
>   3. Re: Moth Sperm. (Geoff McAuliffe)
>   4. can someone please explain lyses (JCarpenter764 <@t> aol.com)
>   5. (no subject) (Wright, Barbara (SPRI 2))
>   6. CD25 (Dawson, Glen)
>   7. rhodamine auto-fluor. problem (Bonnie P Whitaker)
>   8. (no subject) (JCarpenter764 <@t> aol.com)
>   9. RE: (no subject) (Barry R Rittman)
>  10. Reality Check (Joe Nocito)
>  11. Re: CD23 and BCL-6 on B5 fixed tissue (Hadi Yaziji)
>  12. RE: (no subject) (Smith, Allen)
>
>--__--__--
>
>Message: 1
>Date: Fri, 24 Oct 2003 10:46:00 -0700 (PDT)
>From: amosbrooks <amosbrooks <@t> earthlink.net>
>Reply-To: amosbrooks <@t> earthlink.net
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Re: Histonet digest, Vol 1 #100 - 15 msgs
>
>Ronnie,
>     We use Novocastra's CD25 (NCL CD25 305) at 1:100. We pretreat with
>hot
>citrate buffer (HIER) and detect with Envision.
>Amos Brooks
>
>
>
>From: "Houston, Ronnie" <Ronnie_Houston <@t> bshsi.com>
>To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
>Date: Thu, 23 Oct 2003 12:58:03 -0400
>Subject: [Histonet] CD 25
>
>
>Is anyone using CD25 on formalin-fixed, paraffin-
>embedded human tissue? What clone and source are available?
>
>Thanks
>Ronnie Houston
>Regional Histology Operations Manager
>Bon Secours HealthPartners Laboratories
>5801 Bremo Road
>Richmond, VA 23226
>(804) 287 7972
>ronnie_houston <@t> bshsi.com
>
>
>--__--__--
>
>Message: 2
>Date: Fri, 24 Oct 2003 14:09:07 -0400
>From: Dana Settembre <settembr <@t> umdnj.edu>
>To: Ronnie_Houston <@t> bshsi.com, histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] CD 25
>
>Hello Ronnie,
>I use CD25 (Interleukin-2 Receptor) from Novocastra Laboratories which
>is distributed by Vector Laboratories in Burlingame, CA.
>I use in on FFPE human tissue.  Their clone is  4C9.  Their catalog
>number is NCL-CD25-305.
>Their spec sheet recommends a tonsil for a positive control.
>I use it at 1:50 with Dako's Target Retrieval Solution.
>Good Luck.
>Dana Settembre
>University Hospital-UMDNJ
>Newark, NJ
>
>
>  
>
>>>>"Houston, Ronnie" <Ronnie_Houston <@t> bshsi.com> 10/23/2003 9:58:03 AM
>>>>
>>>>        
>>>>
>
>Is anyone using CD25 on formalin-fixed, paraffin-
>embedded human tissue? What clone and source are available?
>
>Thanks
>Ronnie Houston
>Regional Histology Operations Manager
>Bon Secours HealthPartners Laboratories
>5801 Bremo Road
>Richmond, VA 23226
>(804) 287 7972
>ronnie_houston <@t> bshsi.com 
>
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>--__--__--
>
>Message: 3
>Date: Fri, 24 Oct 2003 14:14:46 -0400
>From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
>To: Bruce Abaloz <brucea <@t> unimelb.edu.au>
>Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>,
> histonet-admin <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Moth Sperm.
>
>Can you see the nucleus? If so, I don't understand the problem. Perhaps 
>you are trying to stain the whole nucleated sperm one color and the 
>whole anculeate sperm another? Otherwise, just stain for something found
>
>in a nucleus and not in the cytoplasm.
>
>Geoff
>
>Bruce Abaloz wrote:
>
>  
>
>>Hello Everyone,
>>my name is Kathryn Mcnamara & I am a PhD student at the University of
>>    
>>
>Melbourne, Australia. I am examining the sperm of moths. Moths have 2
>types of sperm a nucleated (eupyrene) sperm and an anucleated (apyrene)
>sperm. 
>  
>
>>I was/AM hoping to find something that would only stain the nucleated
>>    
>>
>sperm so that the two can be easily differentiated with the one stain.
>If you could help me it would be very much appreciated. THANKS in
>advance,
>  
>
>>Kathryn McNamara
>>
>>Department of Zoology
>>University of Melbourne
>>Victoria, Australia 3010
>>
>>
>> 
>>
>>    
>>
>
>  
>
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