[Histonet] Mouse Embryo Processing

[JBurrill <@t> criver.com]

Hi Stephanie,
It depends on what you are looking for.  If you are interested in multiple 
organs the most consistent way of processing is trimming the embryo into 
three mid-siggital sections for gestational days 15-18.  Two on either 
side of the umbilicus and the umbilicus section.  I have processed a lot 
of embryos and found this is the most consistent reproducible processing 
method.  I would also agree that you need more time in the xylene, we use 
3 stations for 45 min each. 


Jason D. Burrill, B.A., HT(ASCP)
Senior Supervisor, Histology 
Charles River Laboratories
251 Ballardvale Street
Wilmington, MA 01887
ph:   978-658-6000 ext 1652
fax:  978-988-8793
E-mail:  jburrill <@t> criver.com
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