<br><font size=2 face="sans-serif">Hi Stephanie,</font>
<br><font size=2 face="sans-serif">It depends on what you are looking for. If you are interested in multiple organs the most consistent way of processing is trimming the embryo into three mid-siggital sections for gestational days 15-18. Two on either side of the umbilicus and the umbilicus section. I have processed a lot of embryos and found this is the most consistent reproducible processing method. I would also agree that you need more time in the xylene, we use 3 stations for 45 min each. <br>
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<br>
Jason D. Burrill, B.A., HT(ASCP)<br>
Senior Supervisor, Histology <br>
Charles River Laboratories<br>
251 Ballardvale Street<br>
Wilmington, MA 01887<br>
ph: 978-658-6000 ext 1652<br>
fax: 978-988-8793<br>
E-mail: jburrill@criver.com</font>