[Histonet] IHC QC's

[Horn, Hazel V]

For those of us who will not be attending NSH will you send us a copy of the
meetings minutes? 

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912 

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> msn.com] 
Sent: Tuesday, October 14, 2003 11:01 AM
To: ploykasek <@t> phenopath.com; HornHV <@t> archildrens.org;
histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] IHC QC's


IHC QC will be a topic discussed at the IHC Resource Group committee meeting

during the NSH S/C in Louisville.  The committee meeting will be on Saturday

at 5:30 PM.  I invite all those attending the NSH S/C with an interest in 
IHC to attend this meeting.
Best regards,
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg <@t> msn.com
wk email pruegg <@t> colobio.com

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----Original Message Follows----
From: Patti Loykasek <ploykasek <@t> phenopath.com>
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,histonet 
<histonet <@t> pathology.swmed.edu>
Subject: Re: [Histonet] IHC QC's
Date: Mon, 13 Oct 2003 14:18:00 -0700

There was in fact, a post from Nick Kirk on running a positive control with
each case. I do realize the CAP requirements and am familiar with the
checklist.

Patti Loykasek
Phenopath Laboratories
Seattle, WA
 >
 >
 > I don't think anyone said a POSTIVE control should be run with each 
slide.
 > We were talking about negative controls, I believe.
 > I just copied and pasted from the latest CAP survey in another email.  >
>  > Hazel Horn, HT/HTL (ASCP)  > Histology Supervisor  > Arkansas
Children's Hospital  >  > Phone - 501.364.4240  > Fax - 501.364.3912  >>  >>
-----Original Message-----  >> From: Patti Loykasek
[mailto:ploykasek <@t> phenopath.com]  >> Sent: Monday, October 13, 2003 10:52 AM
>> To: histonet  >> Subject: [Histonet] IHC QC's  >>  >> I'm glad that
everyone is so concerned with both negative and positive 
IHC
 >> controls. There is certainly more than one side to this issue. I will 
say
 >> that I don't think a positive QC on every slide is absolutely necessary,

for
 >> many reasons. If the QC is rare & precious, then it is a waste of 
resources.
 >> As is running a negative control for every possible technique 
permutation on
 >> small amounts of tumor. I would rather have slides with tumor left for
>> additional studies than have wasted tumor sections on 4-6 negative 
controls.
 >> You can always evaluate non-specific staining on slides that have had an
>> antibody applied & that are negative with that antibody. The CAP is 
specific
 >> that positive controls be used for each antibody - see  CAP checklist
>> ANP.22550. They do not specify for each slide. Since positive QC's 
should be
 >> kept filed for the same number of years as the patient slide & records, 
it
 >> should be possible to pull a QC slide from the IHC run for a particular
>> slide. In the CAP comment on ANP.22550, the use of internal QC's is also
>> mentioned. Although there are many ways of dealing with the issue of 
QC's,
 >> I'm sure we all want to do what is prudent, abide by the regulations, 
and
 >> increase the level of patient care.
 >> Just my 2 cents worth.
 >>
 >> Patti Loykasek
 >> Phenopath Laboratories
 >> Seattle, WA
 >
 > The information contained in this message may be privileged and 
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 > Thank you. Arkansas Children's Hospital
 >

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The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital