[Histonet] IHC QC's

Tue Oct 14 11:00:52 CDT 2003

IHC QC will be a topic discussed at the IHC Resource Group committee meeting 
during the NSH S/C in Louisville.  The committee meeting will be on Saturday 
at 5:30 PM.  I invite all those attending the NSH S/C with an interest in 
IHC to attend this meeting.
Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg <@t> msn.com
wk email pruegg <@t> colobio.com

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----Original Message Follows----
From: Patti Loykasek <ploykasek <@t> phenopath.com>
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,histonet 
<histonet <@t> pathology.swmed.edu>
Subject: Re: [Histonet] IHC QC's
Date: Mon, 13 Oct 2003 14:18:00 -0700

There was in fact, a post from Nick Kirk on running a positive control with
each case. I do realize the CAP requirements and am familiar with the
checklist.

Patti Loykasek
Phenopath Laboratories
Seattle, WA
 >
 >
 > I don't think anyone said a POSTIVE control should be run with each 
slide.
 > We were talking about negative controls, I believe.
 > I just copied and pasted from the latest CAP survey in another email.
 >
 >
 > Hazel Horn, HT/HTL (ASCP)
 > Histology Supervisor
 > Arkansas Children's Hospital
 >
 > Phone - 501.364.4240
 > Fax - 501.364.3912
 >>
 >> -----Original Message-----
 >> From: Patti Loykasek [mailto:ploykasek <@t> phenopath.com]
 >> Sent: Monday, October 13, 2003 10:52 AM
 >> To: histonet
 >> Subject: [Histonet] IHC QC's
 >>
 >> I'm glad that everyone is so concerned with both negative and positive 
IHC
 >> controls. There is certainly more than one side to this issue. I will 
say
 >> that I don't think a positive QC on every slide is absolutely necessary, 
for
 >> many reasons. If the QC is rare & precious, then it is a waste of 
resources.
 >> As is running a negative control for every possible technique 
permutation on
 >> small amounts of tumor. I would rather have slides with tumor left for
 >> additional studies than have wasted tumor sections on 4-6 negative 
controls.
 >> You can always evaluate non-specific staining on slides that have had an
 >> antibody applied & that are negative with that antibody. The CAP is 
specific
 >> that positive controls be used for each antibody - see  CAP checklist
 >> ANP.22550. They do not specify for each slide. Since positive QC's 
should be
 >> kept filed for the same number of years as the patient slide & records, 
it
 >> should be possible to pull a QC slide from the IHC run for a particular
 >> slide. In the CAP comment on ANP.22550, the use of internal QC's is also
 >> mentioned. Although there are many ways of dealing with the issue of 
QC's,
 >> I'm sure we all want to do what is prudent, abide by the regulations, 
and
 >> increase the level of patient care.
 >> Just my 2 cents worth.
 >>
 >> Patti Loykasek
 >> Phenopath Laboratories
 >> Seattle, WA
 >
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 > Thank you. Arkansas Children's Hospital
 >

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